Sodium Hydrogen-Carbonate Agar Base – 250g for Bacillus anthracis Virulence Test
Sodium Hydrogen-Carbonate Agar Base is a specialized medium designed for the virulence testing of Bacillus anthracis. By supplementing with sterile horse serum, the medium enables differentiation between virulent and avirulent anthrax strains based on colony morphology and capsule formation.
Product Information
Product Name: Sodium Hydrogen-Carbonate Agar Base
Pack Size: 250g per bottle
Form: Dehydrated culture medium powder
Storage: Store at room temperature
Shelf Life: 3 years
Applications
Virulence testing of Bacillus anthracis
Differentiation of capsule-forming (virulent) and non-capsule-forming (avirulent) strains
Public health, veterinary, and microbiological research
Key Features
Formulated with sodium hydrogen-carbonate for CO₂-enriched culture conditions
Enables reliable virulence assessment of anthrax strains
Supports clear differentiation of colony morphology under microscopy
Medium Composition (per liter)
Peptone: 10.0 g
Beef Extract: 5.0 g
Sodium Chloride: 5.0 g
Sodium Hydrogen-Carbonate: 7.5 g
Agar: 15.0 g
Preparation Instructions
Suspend 4.25 g of the medium in 100 ml distilled or deionized water.
Sterilize by autoclaving at 121℃ for 15 minutes.
Cool to 45–50℃, then aseptically add 3–5 ml of sterile horse serum.
Mix thoroughly and pour into sterile Petri dishes.
Incubate at 37℃ under 10–20% CO₂ atmosphere for 24 hours.
