SOB Medium – 250g, for Genetic Engineering E. coli Cultivation

Our SOB Medium is a specialized culture medium formulated to support the efficient cultivation of genetic engineering Escherichia coli (E. coli). Each 250g bottle delivers consistent performance, making it ideal for laboratory research, biotech applications, and genetic engineering workflows where reliable microbial growth is critical. With a 3-year shelf life under room temperature storage, it offers long-term convenience and cost-effectiveness for your lab operations.

Product Specifications

• Product Name: SOB Medium

• Pack Size: 250g/bottle

• Storage Condition: Room temperature

• Shelf Life: 3 years

• Target Application: Cultivation of genetic engineering Escherichia coli (E. coli)

• pH: 7.0 ± 0.2 (at 25℃)

Complete Medium Formula (Per Liter)

• Tryptone: 20.0g

• Yeast Extract: 5.0g

• Sodium Chloride (NaCl): 0.5g

• Potassium Chloride (KCl): 0.186g

• Magnesium Sulfate (MgSO₄): 2.4g

Usage Instructions

1. Weigh 28.0g of the SOB Medium powder into 1 liter of distilled water or deionized water.

2. Heat and boil the mixture until the powder is completely dissolved.

3. Dispense the solution into appropriate containers (e.g., flasks, test tubes) as needed.

4. Autoclave at 121℃ for 15 minutes to sterilize.

5. Allow the medium to cool to the desired temperature before use.