SCDLP Agar Base for Total Bacterial Count

SCDLP Agar Base (Soya Casein Digest Lecithin Polysorbate Agar Base) is a dehydrated solid culture medium designed for total bacterial count determination and general bacterial cultivation.

This medium is commonly used as a base formulation and requires the addition of Polysorbate 80 (Tween 80) prior to sterilization. Its balanced nutrient composition supports reliable colony development and consistent performance in routine microbiological analysis.

The powdered format ensures easy preparation, stable quality, and extended shelf life, making it suitable for microbiology laboratories performing quantitative bacterial analysis and routine culture work.

🔬 Applications

• Total bacterial count determination

• General bacterial cultivation

• Routine microbiological testing

• Sample analysis requiring solid enrichment media

📦 Product Specifications

• Product Name: SCDLP Agar Base

• English Name: Soya Casein Digest Lecithin Polysorbate Agar Base

• Form: Dehydrated powder

• Package Size: 250 g / bottle

• Storage Condition: Room temperature, dry place

• Shelf Life: 3 years

🧾 Medium Composition (per liter)

• Pancreatic Digest of Casein – 17.0 g

• Enzymatic Digest of Soybean Meal – 3.0 g

• Dipotassium Phosphate – 2.5 g

• Sodium Chloride – 5.0 g

• Dextrose – 2.5 g

• Lecithin – 1.0 g

• Agar – 15.0 g

Final pH: 7.2 ± 0.2 (25 °C)

⚙️ Directions for Use

1. Suspend 46.0 g of the medium in 1 liter of distilled or deionized water.

2. Add 7.0 g of Polysorbate 80 (Tween 80) per liter
   (or 0.7 g per 100 mL of medium).

3. Heat to boiling and maintain gentle boiling for at least 1 minute until completely dissolved.

4. Dispense into suitable containers.

5. Sterilize by autoclaving at 121 °C for 20 minutes.

6. Allow to cool slightly, mix gently, and prepare plates as required.