Salmonella Shigella Enrichment Broth – Selective Growth Medium, 250g
Salmonella Shigella Enrichment Broth is a selective enrichment medium designed for the isolation and enrichment of Salmonella and Shigella species. With a balanced formulation of peptones, bile salts, and selective inhibitors, this medium provides an optimal environment for target bacteria while suppressing competing organisms. It is widely used in food safety, clinical diagnostics, and microbiological research.
Product Information
Product Name: Salmonella Shigella Enrichment Broth
Pack Size: 250g per bottle
Form: Dehydrated powder
Storage: Room temperature
Shelf Life: 3 years
Applications
Selective enrichment of Salmonella spp.
Selective enrichment of Shigella spp.
Food microbiology and safety testing
Clinical and public health laboratories
Research in pathogenic bacteria isolation
Composition (per liter)
Proteose – 2.0g
Peptone – 8.0g
Beef Extract – 3.5g
Yeast Extract – 2.0g
Dextrose – 2.0g
Sodium Thiosulfate – 10.0g
Sodium Citrate – 10.0g
Magnesium Sulfate – 0.7g
Sulfites – 0.7g
Disodium Hydrogen Phosphate – 4.0g
Monosodium Phosphate – 0.1g
Bile Salt – 5.5g
Sodium Desoxycholate – 1.5g
Brilliant Green – 0.005g
pH 7.1 ± 0.1 (25°C)
Preparation Instructions
Suspend 50.0g of Salmonella Shigella Enrichment Broth in 1L distilled or deionized water.
Heat with boiling until completely dissolved.
Boil in a water bath for 2–3 minutes.
Dispense into sterile test tubes.
Note: This medium does not require autoclaving.
Safety Notes
For laboratory use only.
Handle pathogenic organisms under appropriate biosafety conditions.
