Sabouraud’s Agar Medium – 250g | Fungal Culture & Testing

Classic Acidic Medium for Fungal Cultivation and Antimicrobial Testing

Sabouraud’s Agar Medium provides optimal conditions with high glucose concentration (40g/L) and acidic pH (5.6) to support robust fungal growth while inhibiting bacterial contamination. The high agar content (20g/L) ensures excellent gel strength for reliable plating applications.

Key Applications:

– Fungicidal efficacy testing

– Antifungal product evaluation

– Clinical mycology diagnostics

– Environmental fungal monitoring

– Pharmaceutical preservative testing

– Food and cosmetic spoilage detection

Technical Specifications:

– Formula (per liter):

– Peptone: 10.0g

– Dextrose: 40.0g

– Agar: 20.0g

– pH: 5.6 ± 0.2 (25°C)

– Packaging: 250g/bottle

– Storage: Room temperature (15-25°C)

– Shelf Life: 3 years

Preparation Protocol:

1. Suspend 70.0g in 1L distilled water

2. Heat with continuous stirring until completely dissolved

3. Sterilize at 115°C for 30 minutes

4. Swirl during cooling to prevent settling

Optional Enhancement:

For increased bacterial inhibition, cool to approximately 50°C and add chloramphenicol (10mg per 100ml) aseptically before pouring plates.

Quality Features:

– High dextrose content supports rapid fungal growth

– Acidic pH creates selective environment against bacteria

– 20g/L agar ensures optimal surface for colony development

– Proven reliability in clinical and industrial applications