Key Features & Benefits:
- Liquid Format (Broth): Specifically designed for fungicidal assays, sterility testing, and preparing fungal suspensions. Contains no solidifying agent (agar).
- Standardized Formulation: Follows the classical Sabouraud composition (high glucose, low pH) to support the growth of dermatophytes, yeasts, and filamentous fungi.
- Flexible Supplementation: Can be easily supplemented with antibiotics like chloramphenicol to create a more selective environment for primary isolation from non-sterile samples.
- Easy Preparation: Simple dissolution and sterilization protocol.
Product Specifications
| Specification | Details |
|---|---|
| Product Type | Dehydrated Culture Media (Broth Base) |
| Quantity | 250g (Prepares exactly 5.0 Liters of broth) |
| Application | Fungal culture; Fungicidal testing; Enrichment |
| Format | Broth (Liquid medium) |
| Storage | Room temperature (Stable for 3 years) |
| Selective Properties | Low pH 5.6 |
Composition (per liter)
- Peptone: 10.0g
- Dextrose (Glucose): 40.0g
Final pH: 5.6 ± 0.2 at 25°C
Instructions for Preparation
- Suspend 50.0g of the powder in 1 liter of distilled or deionized water. Warm gently to dissolve completely.
- Dispense into final containers (e.g., tubes, flasks).
- Sterilize by autoclaving at 115°C for 30 minutes.
- Cool before use.
- Optional Selective Supplement: For inhibiting bacteria, aseptically add chloramphenicol (10mg) per 100ml of sterile broth cooled to below 50°C.