
Rose Bengal Agar Medium | Fungal Isolation & Enumeration
Product Name: Rose Bengal Agar Medium
Application: Used for fungal isolation and enumeration.
Packing Size: 250 g/bottle
Storage: Room temperature
Shelf Life: 3 years
Formula (per liter):
Peptone – 5.0 g
Dextrose – 10.0 g
Monopotassium Phosphate – 1.0 g
Magnesium Sulfate – 0.5 g
Rose Bengal – 0.0133 g
Agar – 14.0 g
Final pH: 6.0 ± 0.2 (at 25°C)
Preparation Instructions:
Dissolve 30.5 g of the powder in 1 L of distilled or deionized water. Heat to boiling until fully dissolved, dispense, and sterilize by autoclaving at 121°C for 15 minutes or at 115°C for 30 minutes. Mix well after sterilization to prevent agar sedimentation. Medium is ready for use.



