Key Features & Benefits:
- Enhanced Counting Accuracy: Rose Bengal restricts the spreading of mold colonies, keeping them compact and discrete for precise enumeration.
- Visual Differentiation: Colonies take up the Rose Bengal dye, appearing pink to red, which improves contrast and makes counting easier.
- Bacterial Suppression: Effectively inhibits most bacterial growth without the need for added antibiotics.
- Standardized Formulation: Consistent composition ensures reliable and reproducible results in quantitative fungal assays.
Product Specifications
| Specification | Details |
|---|---|
| Product Type | Dehydrated Culture Media |
| Quantity | 250g (Prepares approximately 8.2 Liters of medium) |
| Application | Fungal isolation and enumeration |
| Storage | Room temperature (Stable for 3 years) |
| Selective Agent | Rose Bengal (0.0133g/L) |
Composition (per liter)
| Component | Amount |
|---|---|
| Peptone | 5.0g |
| Dextrose (Glucose) | 10.0g |
| Monopotassium phosphate (KH₂PO₄) | 1.0g |
| Magnesium Sulfate (MgSO₄) | 0.5g |
| Rose Bengal | 0.0133g |
| Agar | 14.0g |
Final pH: 6.0 ± 0.2 at 25°C
Instructions for Preparation
- Suspend 30.5g of the powder in 1 liter of distilled or deionized water.
- Heat and boil to dissolve the medium completely.
- Dispense into final containers.
- Sterilize by autoclaving at 121°C for 15 minutes or 115°C for 30 minutes.
- After sterilization, gently swirl the vessel to prevent agar sedimentation before the medium solidifies.
- Cool and pour into sterile petri dishes.
Typical Colony Characteristics
| Control Strain | Growth | Colony Appearance |
|---|---|---|
| Candida albicans | Good | Pink to red colonies |
| Aspergillus niger | Good | Dark spores, pink background |
| Saccharomyces cerevisiae | Good | White to pink colonies |
| Escherichia coli | Inhibited | No growth |