Rogosa SL Agar – Selective Medium for Lactobacillus, 250g

Rogosa SL Agar is a selective culture medium designed for the detection and cultivation of Lactobacillus species in oral, vaginal, and fecal samples. Its selective composition allows robust Lactobacillus growth while inhibiting non-target microorganisms, making it ideal for microbiological testing and probiotic research.

Product Information

• Product Name: Rogosa SL Agar

• Form: Dehydrated powder

• Pack Size: 250g per bottle

• Storage: Room temperature

• Shelf Life: 3 years

Medium Composition (per liter)

• Tryptone – 10.0 g

• Yeast Extract – 5.0 g

• Dextrose – 10.0 g

• Arabinose – 5.0 g

• Sucrose – 5.0 g

• Sodium Acetate – 15.0 g

• Monopotassium Phosphate – 6.0 g

• Ammonium Citrate – 2.0 g

• Magnesium Sulfate – 0.57 g

• Manganese Sulfate – 0.12 g

• Ferrous Sulfate – 0.03 g

• Agar – 15.0 g

• pH: 5.4 ± 0.2 (25℃)

Preparation Instructions

1. Dissolve 73.7 g of the medium in 1 liter of distilled or deionized water.

2. Boil for 1 minute until fully dissolved.

3. Add 1 ml Tween 80 and 1.32 ml glacial acetic acid, mix thoroughly.

4. Adjust pH to 5.4 ± 0.2 (25℃).

5. Continue boiling for 2–3 minutes. Do not autoclave.

6. Cool and pour plates for use.

Applications

• Selective cultivation and detection of Lactobacillus spp.

• Microbiological analysis of oral, vaginal, and fecal samples

• Probiotic research and quality control