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Ready-to-Use TSA Contact Plate with Penicillinase – Soybean-Casein Digest Agar, 65 mm, 10 Plates/Pack, Sterile Surface Microbial Monitoring Media

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Ready-to-use Soybean-Casein Digest Agar (TSA) contact plates supplemented with penicillinase (beta-lactamase), designed for surface microbial environmental monitoring of equipment, work surfaces, personnel and packaging materials in controlled environments. Produced in accordance with USP, EP and GB16294 requirements.

Key Features

  • Ready-to-use sterile contact plate: autoclaved, aseptically filled and gamma-irradiated for consistent sterility.
  • Penicillinase (1,000,000 IU/L): inactivates residual penicillin in samples, helping to prevent false-negative counts during surface monitoring in antibiotic-manufacturing and clean environments.
  • Broad growth support for common environmental indicator organisms.
  • Flat, crack-free, pale-yellow agar surface, raised to an appropriate height above the plate rim for reliable contact sampling.
  • Convenient 65 mm size, 15–20 mL fill; 10 plates per pack in triple-layer vacuum packaging for cleanroom transfer.

Composition (per 1000 mL)

  • Tryptone (pancreatic digest of casein): 15 g
  • Soya peptone (papain digest of soybean): 5 g
  • Sodium chloride: 5 g
  • Agar: 15 g
  • Penicillinase: 1,000,000 IU
  • Distilled water: 1000 mL
  • Final pH: 7.3 ± 0.2 at 25 °C

Intended Use

For determining surface microbial load on equipment, workshop/work areas, personnel and packaging materials in cleanrooms and controlled manufacturing environments. This is an environmental monitoring medium for laboratory use only. It is not a medical device and is not intended for diagnosis or treatment of disease.

Performance

  • Appearance: pale yellow; agar raised slightly above the rim; smooth surface; free from cracks, visible impurities and air bubbles.
  • Physical & chemical: pH within specification; gel strength 500–650 g/cm².
  • Sterility & moisture retention: no bacterial growth after 5 days at 30–35 °C; no significant dehydration.
  • Growth promotion: supports good growth with typical colony morphology for Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis and Enterococcus faecalis (50–100 CFU).

How to Use

  1. Remove the contact plate from refrigerated storage and allow it to reach room temperature.
  2. Bring the intact package into the sampling area, gradually removing the outer two layers; open the final vacuum layer inside the operating room.
  3. Open the lid and press the medium firmly onto the surface to be sampled; if needed, press gently on the centre of the base for about 10 seconds, lift slowly, and replace the lid from one side.
  4. Mark the frosted area with sampling location, time and sampler.
  5. Wipe the tested area with a disinfectant or sporicide to remove medium residue.
  6. Remove the plate from the cleanroom and incubate inverted at the specified temperature and time.
  7. Observe and count colonies.

Storage & Shelf Life

Store at 2–8 °C, protected from light. Shelf life: 60 days from date of manufacture. Do not use if the plate is cracked, dried out or contaminated.

Packaging

65 mm contact plate, 15–20 mL fill, 10 plates per pack; triple-layer vacuum packaging.

Safety & Disposal

Single-use product. Autoclave before disposal. Handle as laboratory material in accordance with your facility’s biosafety procedures.

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