Rappaport-Vassiliadis Medium, Semisolid Modification (MSRV Broth Base) – Selective Medium for Salmonella, 250g

Modified Semisolid Rappaport-Vassiliadis Medium (MSRV) is a selective culture medium designed for the isolation and differentiation of Salmonella species. The semisolid formulation allows for enhanced motility testing while selectively inhibiting competing microorganisms through the presence of malachite green and magnesium chloride. This medium is widely used in food microbiology, clinical diagnostics, and public health laboratories.

Product Information

• Product Name: Modified Semisolid Rappaport-Vassiliadis Medium (MSRV)

• Pack Size: 250g per bottle

• Form: Dehydrated powder

• Storage: Room temperature

• Shelf Life: 3 years

Applications

• Selective isolation of Salmonella spp.

• Food microbiology and pathogen detection

• Clinical microbiology laboratories

• Public health surveillance

Composition (per liter)

• Tryptose – 4.59g

• Casein Hydrolysate Acid – 4.59g

• Sodium Chloride – 7.34g

• Monopotassium Phosphate – 1.47g

• Magnesium Chloride Anhydrous – 10.93g

• Malachite Green Oxalate – 0.037g

• Agar – 2.7g

• pH 5.2 ± 0.2 (25°C)

Preparation Instructions

1. Suspend 31.6g of MSRV Base in 1L distilled or deionized water (adjust volume proportionally if preparing less or more).

2. Heat to boiling for 1 minute until completely dissolved.

3. Cool to 50°C.

4. Under aseptic conditions, add 1 vial novobiocin solution (2mg) per 100ml medium.

5. Mix well, dispense, and allow to solidify.

6. Note: Do not autoclave this medium.

Safety Notes

• For laboratory use only.

• Handle pathogenic organisms under biosafety conditions.