R2A Agar – Low Nutrient Medium for Pharmaceutical Water Testing
Pharmaceutical-Grade R2A Agar – Gold Standard for Water System Bioburden Monitoring
Meet pharmacopeial requirements with our precision-formulated R2A Agar – the essential low-nutrient medium designed for accurate recovery of stressed microorganisms in purified water, WFI, and process fluids. Trusted by QC labs to comply with USP <61>, EP 2.6.12, and FDA water testing protocols.
Critical Applications:
✔️ Potable Water Testing – Aerobic Counts in Purified/WFI Systems
✔️ Environmental Monitoring – Low-Bioburden Area Assessment
✔️ Stressed Microbe Recovery – Injured Bacteria & Oligotrophs
✔️ Pharmaceutical QC – Manufacturing Process Validation
Scientifically Validated Formula (per liter):
| Component | Quantity | Function |
|—————————-|———-|——————————|
| Yeast Extract | 0.5g | Vitamin/Coenzyme Source |
| Proteose Peptone | 0.5g | Polypeptide Nutrients |
| Casein Hydrolysate | 0.5g | Amino Acid Supplement |
| Dextrose | 0.5g | Energy Substrate |
| Soluble Starch | 0.5g | Slow-Release Carbon |
| Dipotassium Phosphate | 0.3g | pH Buffer |
| Magnesium Sulfate (Anhyd.) | 0.024g | Enzyme Cofactor |
| Sodium Pyruvate | 0.3g | Oxidative Stress Protection*|
| High-Gel Agar | 15.0g | Solid Matrix Formation |
pH: 7.2 ± 0.2 (25°C) – Optimized for chlorine-damaged microbes
Pyruvate neutralizes peroxides to resuscitate injured bacteria
Validated Preparation Protocol:
Dissolve 18.1g powder in 1L Type I water
Boil 1 minute with constant stirring
Autoclave: 121°C/15 min (Do not over-sterilize)
Cool to 45-50°C before pouring plates
