Pseudomonas Isolation Agar – Selective Medium with Irgasan for Pseudomonas Detection (250g)

Pseudomonas Isolation Agar is a selective medium formulated for the detection and isolation of Pseudomonas species from clinical, food, water, and environmental samples.

The medium contains peptone as a nutrient source, while magnesium chloride and potassium sulfate enhance pigment production such as pyocyanin and fluorescein in Pseudomonas aeruginosa. The addition of Irgasan (triclosan) provides selectivity by suppressing the growth of most Gram-positive and other non-target bacteria.

This medium is widely applied in microbiological testing, environmental monitoring, and quality control laboratories for the reliable isolation of Pseudomonas.

Complete Formula (per liter):

• Peptone: 20.0 g

• Magnesium Chloride (MgCl₂): 1.4 g

• Potassium Sulfate (K₂SO₄): 10.0 g

• Irgasan: 0.025 g

• Agar: 13.6 g

• Final pH: 7.0 ± 0.2 (25°C)

Preparation & Instructions:

1. Suspend 45.0 g of the medium in 1 L distilled or deionized water containing 2% glycerol.

2. Heat with constant stirring and boil for at least 1 minute until completely dissolved.

3. Dispense into suitable containers.

4. Sterilize by autoclaving at 121°C for 15 minutes.

5. Allow to cool and solidify before inoculation.

Packaging & Storage:

• 250 g per bottle

• Shelf life: 3 years

• Storage: Store in a cool, dry place at room temperature, tightly sealed