Product Specifications
- Product Name: Pseudomonas Cepacia Agar Base
- Alternative Name: Burkholderia cepacia Agar Base / PC Agar
- Format: Powder Medium
- Net Weight: 250 g / bottle
- Storage: Store in a cool and dry place at room temperature
- Shelf Life: 3 years
- Application: Selective isolation of Burkholderia cepacia
Composition (g/L)
Sodium Pyruvate ………………………………. 7.0
Peptone ………………………………………….. 5.0
KH₂PO₄ ……………………………………………. 4.4
Yeast Extract ……………………………………. 4.0
Bile Salts …………………………………………. 1.5
Na₂HPO₄ ………………………………………….. 1.4
(NH₄)₂SO₄ ………………………………………… 1.0
MgSO₄ ……………………………………………… 0.2
Phenol Red ……………………………………….. 0.02
Fe(NH₄)₂(SO₄)₂·6H₂O …………………………… 0.01
Crystal Violet ……………………………………. 0.001
Agar ……………………………………………….. 12.0
pH 6.2 ± 0.2 (25°C)
Preparation Instructions
- Weigh 36.5 g of the medium powder.
- Dissolve in 1000 mL distilled or deionized water.
- Heat and boil until the medium is completely dissolved.
- Dispense into suitable containers.
- Sterilize at 121°C for 15 minutes.
- After sterilization, mix well and cool to 50–55°C.
- Under aseptic conditions, add the following supplements to each 100 mL of medium:
- Polymyxin B solution
- Hydroxythiazolyl penicillin solution
- Gentamicin solution
- Mix gently and pour into sterile Petri dishes.
Applications
- Isolation of Burkholderia cepacia
- Cosmetic microbiology testing
- Pharmaceutical microbiological quality control
- Environmental microbiology monitoring
- Research laboratories