Pseudomonas Agar Medium (PAP) – For Pyocyanin Determination in Pseudomonas aeruginosa (250g)

Pseudomonas Agar Medium (PAP) is a selective medium designed for the determination of pyocyanin production by Pseudomonas aeruginosa. Pyocyanin is a characteristic blue-green pigment that serves as an important marker for the identification and differentiation of P. aeruginosa.

This medium enhances pigment production under controlled conditions and is widely used in:

• Clinical microbiology laboratories

• Research studies on Pseudomonas aeruginosa

• Public health and environmental monitoring

• Microbial physiology and biochemistry experiments

Composition (per liter):

• Pancreatic Digest of Gelatin – 20.0 g

• Magnesium Chloride – 1.4 g

• Potassium Sulfate – 10.0 g

• Agar – 15.0 g

• Final pH: 7.2 ± 0.2 (at 25°C)

Preparation & Instructions:

1. Suspend 46.4 g of powder in 1 L distilled or deionized water.

2. Add 1 ml glycerol per 100 ml medium.

3. Heat to boiling until completely dissolved.

4. Dispense into test tubes.

5. Sterilize by autoclaving at 121°C for 15 minutes.

6. Mix thoroughly, cool to 50–60°C, and prepare slant tubes for use.

Packaging & Storage:

• 250 g per bottle

• Shelf life: 3 years

• Storage: Room temperature, tightly closed, away from moisture