Potato Dextrose Broth (250g) for cultivating molds and yeasts in laboratory applications

Potato Dextrose Broth (PDB) – Powder Medium for Yeast and Mold Culture

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Product Overview

Potato Dextrose Broth (PDB) is a dehydrated microbiological culture medium specifically developed for the cultivation and enrichment of yeasts and molds.
It contains potato starch and dextrose, providing a favorable nutrient environment for fungal growth.
This formulation does not contain chloramphenicol, making it suitable for applications where antibiotic-free conditions are required.

The powder format ensures easy preparation, consistent performance, and long shelf life, making it suitable for routine laboratory use.

Key Features

  • Designed for cultivation of yeasts and molds

  • Contains potato starch (4 g/L) and dextrose

  • Antibiotic-free formulation

  • Dehydrated powder for easy storage and preparation

  • Stable performance for microbiology laboratories

Applications

  • Yeast culture

  • Mold culture

  • Fungal growth and enrichment

  • General microbiological research and quality control

Product Specifications

  • Product Name: Potato Dextrose Broth

  • Abbreviation: PDB

  • Form: Dehydrated powder

  • Package Size: 250 g / bottle

  • Storage Condition: Room temperature

  • Shelf Life: 3 years

  • Final pH (25°C): 5.1 ± 0.2

✅ Composition (per liter)

ComponentQuantity
Potato Starch4.0 g
Dextrose20.0 g

✅ Preparation Instructions

  1. Suspend 24.0 g of the product in 1 liter of distilled or deionized water.
  2. Heat gently while stirring until completely dissolved.
  3. Dispense into suitable containers.
  4. Sterilize at 121°C for 15 minutes.
  5. Cool and store for use.

Quality Control

1. Appearance

  • Dehydrated Medium: Light yellow powder.
  • Prepared Medium: Light yellow liquid.

2. Biological CharacteristicsThe following quality control strains were inoculated onto the test medium and incubated at 36～38℃ for 48 hours. The results are as follows:

QC Strain & Strain No.IndicatorQC Criteria
Burkholderia gladioli (Cocovenenans)ATCC 33664Growth Rate(Semi-quantitative)After inoculating < 100 CFU into the medium and incubating at the specified temperature and time, a 100-fold dilution plated on PDA (specific for B. gladioli)** should yield **> 30 CFU.Colony Morphology: Grayish-white or milky white, centrally raised, “sombrero-shaped” (crateriform), with a yellow pigment diffusing into the surrounding medium.

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