Potato Dextrose Agar with Chloramphenicol – 250g | Selective Fungal Enumeration

Selective Medium for Mold and Yeast Enumeration

Potato Dextrose Agar with Chloramphenicol combines natural potato infusion (equivalent to 300g fresh potatoes per liter) with 0.1g/L chloramphenicol to suppress bacterial growth while promoting optimal fungal development for accurate counting.

Key Applications:

– Food and beverage mold and yeast counting

– Pharmaceutical product contamination testing

– Cosmetic microbial limits testing

– Environmental air quality monitoring

– Water system fungal assessment

Technical Specifications:

– Formula (per liter):

– Potato Infusion Powder: 6.0g (from 300g potatoes)

– Dextrose: 20.0g

– Agar: 20.0g

– Chloramphenicol: 0.1g

– pH: 5.6 ± 0.2 (25°C)

– Packaging: 250g/bottle

– Storage: Room temperature (15-25°C)

– Shelf Life: 3 years

Preparation Protocol:

1. Suspend 46.1g in 1L distilled water

2. Dissolve with gentle heating and stirring

3. Sterilize at 121°C for 20 minutes

4. Swirl during cooling to prevent settling

5. Pour plates at approximately 50°C

Quality Features:

– Chloramphenicol effectively inhibits bacterial contamination

– High dextrose concentration supports robust fungal growth

– 20g/L agar ensures optimal gel strength for enumeration

– Acidic pH favors fungal over bacterial development