PLET Agar Base – 250g for Selective Isolation of Bacillus anthracis

PLET Agar Base is a selective culture medium designed for the isolation of Bacillus anthracis. When supplemented with thallium acetate, lysozyme, and polymyxin B, this medium inhibits the growth of most contaminating microorganisms, providing a reliable platform for the selective recovery and differentiation of anthrax bacilli.

Product Information

• Product Name: PLET Agar Base

• Pack Size: 250g per bottle

• Form: Dehydrated culture medium powder

• Storage: Store at room temperature

• Shelf Life: 3 years

Applications

• Selective isolation of Bacillus anthracis

• Microbiological and clinical diagnostic studies

• Public health and veterinary research

Key Features

• Highly selective with lysozyme, polymyxin B, and thallium acetate supplementation

• Based on beef heart infusion and tryptose for optimal bacterial recovery

• Designed to reduce contamination by competing organisms

Medium Composition (per liter)

• Beef heart infusion solids (from 500 g beef heart): 13.0 g

• Tryptose: 10.0 g

• Sodium Chloride: 5.0 g

• Ethylenediaminetetraacetic acid (EDTA): 0.3 g

• Agar: 15.0 g

• Final pH: 7.3 ± 0.2 (25℃)

Preparation Instructions

1. Suspend 43.3 g of the medium in 1 L distilled or deionized water.

2. Heat to boiling until completely dissolved.

3. Dispense into suitable containers and autoclave at 121℃ for 15 minutes.

4. Shake well after sterilization to avoid agar settling at the bottom.

5. Cool to 55℃, then aseptically add (per 100 ml medium):

   • 4 mg thallium acetate

   • 30,000 U lysozyme

   • 3,000 U polymyxin B

6. Mix thoroughly and prepare plates for use.