Product Overview
Pantothenic Acid Assay Medium is a microbiological culture medium formulated for the quantitative determination of pantothenic acid (vitamin B5) in milk powder, food products, and other nutritional samples.
This dehydrated powder medium is commonly used in microbiological assay procedures for vitamin analysis in food testing laboratories, research laboratories, and quality control environments. The medium provides a nutrient composition suitable for microbiological assay systems used in vitamin determination.
The powder medium is dissolved in purified water and prepared according to laboratory procedures before use.
Product Specifications
| Parameter | Description |
|---|---|
| Product Name | Pantothenic Acid Assay Medium |
| Application | Determination of pantothenic acid (Vitamin B5) |
| Packaging | 250 g / bottle |
| Storage | Room temperature |
| Shelf Life | 3 years |
| Form | Dehydrated powder |
Applications
Pantothenic Acid Assay Medium is commonly used for:
- Determination of pantothenic acid (Vitamin B5)
- Nutritional analysis of milk powder and food products
- Microbiological vitamin assay testing
- Food safety laboratories
- Research and quality control laboratories
Medium Composition (per liter)
Glucose — 40.0 g
Sodium Acetate — 20.0 g
KH₂PO₄ — 1.0 g
K₂HPO₄ — 1.0 g
L-Cystine — 0.4 g
L-Tryptophan — 0.1 g
Magnesium Sulfate — 0.4 g
Sodium Chloride — 20.0 mg
Ferrous Sulfate — 20.0 mg
Manganese Sulfate — 20.0 mg
Adenine Sulfate — 20 mg
Guanine Hydrochloride — 20 mg
Uracil — 20.0 mg
Carotene — 0.4 mg
Thiamine Hydrochloride — 0.2 mg
Biotin — 0.8 µg
Pyridoxine Hydrochloride — 0.8 mg
Niacin — 1.0 mg
p-Aminobenzoic Acid — 0.2 mg
Polysorbate Monooleate — 0.1 g
Acid Hydrolyzed Casein (cellulose-free) — 10.0 g
Final pH: 6.7 ± 0.1 (20–25°C)
Preparation Instructions
- Suspend 7.3 g of the medium in 100 ml distilled or deionized water.
- Heat and boil the mixture until the medium is fully dissolved.
- Dispense 5 ml portions into test tubes while mixing to maintain uniform distribution.
- Add appropriate standard solution or sample.
- Adjust the volume to 10 ml with distilled water.
- Sterilize at 121°C for 10 minutes.
- Cool rapidly to room temperature before use.