
Nodule Bacteria Medium II Base for Rhizobium Preservation & Cultivation – 250g
Nodule Bacteria Medium II Base is a specialized microbiological medium designed for the preservation and cultivation of Rhizobium and other nitrogen-fixing bacteria. This medium supports the long-term storage and growth of these important microorganisms, which play a crucial role in soil health and nitrogen fixation. It is commonly used in research and agricultural applications to maintain viable bacterial cultures.
Key Features:
Ideal for Rhizobium preservation and cultivation.
Supports long-term storage of nitrogen-fixing bacteria.
High-quality ingredients ensure consistent and reliable results.
Sterilization ready: Easily prepared for use by autoclaving.
Long shelf life: Stable for up to 3 years at room temperature.
Instructions for Use:
To prepare, dissolve 26.0g of Nodule Bacteria Medium II Base in 1L of distilled or deionized water. Boil until fully dissolved. Sterilize by autoclaving at 121°C for 15 minutes or at 115°C for 30 minutes. After sterilization, gently shake the medium to mix and prevent agar from settling at the bottom. Cool and store until use.
Storage: Store at room temperature, away from direct sunlight. Shelf life: 3 years.
Ingredients per liter:
Mannitol: 10.0g
Yeast Extract: 1.0g
Agar: 15.0g
pH: 7.2 ± 0.2 at 25°C