Nodule Bacteria Medium II Base - For Rhizobium Preservation & Cultivation

Nodule Bacteria Medium II Base for Rhizobium Preservation & Cultivation – 250g

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Nodule Bacteria Medium II Base is a specialized microbiological medium designed for the preservation and cultivation of Rhizobium and other nitrogen-fixing bacteria. This medium supports the long-term storage and growth of these important microorganisms, which play a crucial role in soil health and nitrogen fixation. It is commonly used in research and agricultural applications to maintain viable bacterial cultures.

Key Features:

  • Ideal for Rhizobium preservation and cultivation.

  • Supports long-term storage of nitrogen-fixing bacteria.

  • High-quality ingredients ensure consistent and reliable results.

  • Sterilization ready: Easily prepared for use by autoclaving.

  • Long shelf life: Stable for up to 3 years at room temperature.

Instructions for Use:
To prepare, dissolve 26.0g of Nodule Bacteria Medium II Base in 1L of distilled or deionized water. Boil until fully dissolved. Sterilize by autoclaving at 121°C for 15 minutes or at 115°C for 30 minutes. After sterilization, gently shake the medium to mix and prevent agar from settling at the bottom. Cool and store until use.

Storage: Store at room temperature, away from direct sunlight. Shelf life: 3 years.

Ingredients per liter:

  • Mannitol: 10.0g

  • Yeast Extract: 1.0g

  • Agar: 15.0g

  • pH: 7.2 ± 0.2 at 25°C

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