Nitrogen Utilization Medium – Custom Base for Microbial Nitrogen Metabolism Studies (250g)
Defined Nitrogen Utilization Medium for Microbial Metabolic Profiling
This glucose-based minimal medium (10.0g/L dextrose) provides a standardized platform for evaluating microbial nitrogen source utilization. With balanced phosphate buffer (pH 7.2±0.2) and essential minerals, it enables precise comparison of different nitrogen sources’ effects on microbial growth kinetics and metabolic pathways.
Key Applications:
🔬 Nitrogen Metabolism Research: Study microbial preferences for organic/inorganic N sources
🧫 Industrial Strain Development: Optimize nitrogen utilization in fermentation processes
🧪 Environmental Microbiology: Characterize soil/water microbial N cycling
Technical Highlights:
✅ Nitrogen-Free Base: Contains no interfering nitrogen compounds
✅ Energy-Rich: 10.0g/L glucose supports growth during N source testing
✅ pH-Stabilized: Phosphate buffer maintains 7.2±0.2 through growth phases
✅ Trace Minerals: 0.02g/L FeSO₄ for metalloenzyme synthesis
Preparation Protocol:
Suspend 18.8g in 1L purified water
Add test nitrogen source (typically 10g/L)
Adjust pH to 7.2±0.2 pre-sterilization
Autoclave at 115°C for 30 minutes
Complete Formulation (Per Liter):
KH₂PO₄: 1.36g
Na₂HPO₄: 2.13g
CaCl₂: 0.1g
MgSO₄: 0.2g
NaCl: 5.0g
FeSO₄: 0.02g
Dextrose: 10.0g
