Nicotinic Acid Assay Medium – Culture Medium for Niacin Determination, 250g

Nicotinic Acid Assay Medium is a specialized microbiological medium designed for the quantitative determination of Niacin (Vitamin B3). Its vitamin-controlled composition ensures high sensitivity and reproducibility in vitamin assay applications for microbiology and biochemistry laboratories.

Complete Formula (per liter):

• Casein Acid Hydrolysate (Vitamin Assay Grade): 12.0 g

• Glucose: 40.0 g

• L-Cystine: 0.4 g

• Sodium Acetate: 20.0 g

• DL-Tryptophan: 0.2 g

• Adenine Sulfate: 20 mg

• Guanine Hydrochloride: 20 mg

• Uracil: 20 mg

• Thiamine Hydrochloride: 200 µg

• Riboflavin: 400 µg

• Biotin: 0.8 µg

• Calcium Pantothenate: 200 µg

• Pyridoxine Hydrochloride: 400 µg

• p-Aminobenzoic Acid: 100 µg

• K2HPO4: 1.0 g

• KH2PO4: 1.0 g

• MgSO4: 0.4 g

• NaCl: 20 mg

• FeSO4: 20 mg

• MnSO4: 20 mg

• Final pH: 6.7 ± 0.2 at 25°C

Instructions for Use:

1. Dissolve 7.51 g of medium in 100 ml of distilled or deionized water.

2. Heat to boiling until completely dissolved.

3. Dispense 5 ml per tube.

4. Follow the national standard assay procedure for niacin determination.

5. Note: This product is hygroscopic (easily absorbs moisture); store tightly sealed.

Packaging & Storage:

• 250 g per bottle

• Shelf life: 3 years

• Store in a cool, dry place at room temperature