NAC Agar (Nalidixic Acid Cetrimide Agar Medium) 250g, selective culture medium for Pseudomonas aeruginosa isolation

NAC Agar Medium for Pseudomonas aeruginosa Isolation | Microbiology Culture Medium Powder 250g

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NAC Agar Medium is a dehydrated microbiological culture medium designed for the selective isolation of Pseudomonas aeruginosa in microbiological testing. The powder medium is dissolved in purified water and sterilized before preparation of agar plates used in laboratory analysis.

Product Overview

NAC Agar Medium is a microbiological culture medium formulated for the selective isolation and cultivation of Pseudomonas aeruginosa in laboratory microbiology testing.

This dehydrated agar medium is commonly used in microbiological analysis of microbial ecological products, environmental samples, and laboratory research. The formulation supports the growth of the target microorganism while suppressing competing bacteria.

The medium is supplied as a dehydrated powder and requires dissolution in purified water followed by sterilization before use.


Product Specifications

ParameterDescription
Product NameNAC Agar Medium
ApplicationIsolation of Pseudomonas aeruginosa
Packaging250 g / bottle
StorageRoom temperature
Shelf Life3 years
FormDehydrated powder

Applications

NAC Agar Medium is commonly used for:

  • Isolation of Pseudomonas aeruginosa
  • Microbiology laboratory testing
  • Microbial ecological product testing
  • Environmental microbiology analysis
  • Water microbiology testing
  • Quality control laboratories

Medium Composition (per liter)

Peptone — 20.0 g
Nalidixic Acid — 0.015 g
Magnesium Sulfate — 0.3 g
Dipotassium Phosphate — 0.3 g
Agar — 14.0 g
Cetrimide — 0.3 g


Principle

Peptone provides nutrients required for microbial growth. Magnesium sulfate and dipotassium phosphate supply essential ions in the culture medium. Cetrimide acts as a selective agent that suppresses competing microorganisms. Nalidixic acid functions as a selective antimicrobial component.


Preparation Instructions

  1. Suspend 35 g of the medium in 1000 ml purified water.
  2. Heat the mixture until the medium is completely dissolved.
  3. Dispense the medium into appropriate containers.
  4. Sterilize by autoclaving at 121°C for 15 minutes.
  5. Allow the medium to cool before use.

Note:
Because the medium contains magnesium sulfate, the sterilized medium may appear slightly turbid and small amounts of sediment may be present.

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