MRS Medium without Dextrose – Agar & Broth for Lactic Acid Bacteria Cultivation

MRS Medium without Dextrose – 250g / Bottle

The MRS Medium without Dextrose is a nutrient-rich agar and broth medium specifically formulated for the cultivation of lactic acid bacteria, ideal for research, fermentation, and probiotic studies. This glucose-free formulation allows flexibility for the addition of other nutrients as required for specialized experiments.

Product Information:

• Name: MRS Medium without Dextrose / MRS Agar without Dextrose

• Form: Dehydrated powder

• Pack Size: 250g per bottle

• Storage: Room temperature

• Shelf Life: 3 years

Composition (per liter):

• Peptone – 10.0 g

• Beef Extract – 5.0 g

• Yeast Extract – 4.0 g

• Tween 80 – 1.0 g

• Sodium Acetate·7H₂O – 5.0 g

• K₂HPO₄·7H₂O – 2.0 g

• Ammonium Citrate Tribasic – 2.0 g

• MgSO₄·7H₂O – 0.2 g

• MnSO₄·4H₂O – 0.05 g

• Agar – 15.0 g

• pH: 6.2 ± 0.1 (25℃)

Preparation Instructions:

1. Dissolve 44.3 g of the medium in 1L distilled or deionized water.

2. Heat gently to boiling until fully dissolved.

3. Add other nutrients if required for specific experiments.

4. Dispense into suitable containers.

5. Sterilize by autoclaving at 121℃ for 15 minutes.

6. Shake well after sterilization to prevent agar settling at the bottom.