MRS Broth without Dextrose – Liquid Culture Medium for Lactic Acid Bacteria

MRS Broth without Dextrose – 250g / Bottle

The MRS Broth without Dextrose is a nutrient-rich liquid culture medium specially formulated for the growth and enrichment of lactic acid bacteria, including Lactobacillus species. This formulation excludes dextrose, making it suitable for studies requiring sugar-free conditions, while still supporting robust bacterial growth for probiotic research, fermentation studies, and microbiological applications.

Product Information:

• Name: MRS Broth without Dextrose

• Form: Dehydrated liquid culture medium (powder)

• Pack Size: 250g per bottle

• Storage: Store at room temperature

• Shelf Life: 3 years

Composition (per liter):

• Peptone – 10.0 g

• Beef Extract – 8.0 g

• Yeast Extract – 4.0 g

• Tween 80 – 1.0 g

• Sodium Acetate·H₂O – 5.0 g

• K₂HPO₄·7H₂O – 2.0 g

• Ammonium Citrate Tribasic – 2.0 g

• MgSO₄·7H₂O – 0.2 g

• MnSO₄·4H₂O – 0.05 g

• pH: 6.2 ± 0.1 (25℃)

Preparation Instructions:

1. Dissolve 32.2 g of the medium in 1L distilled or deionized water.

2. Heat gently to boiling until fully dissolved.

3. Dispense into suitable containers.

4. Sterilize by autoclaving at 121℃ for 15 minutes.

5. Cool to room temperature before inoculation.

⚠️ Note: The medium may clump due to Tween 80, but this does not affect its performance.

Applications:

• Sugar-free enrichment and cultivation of lactic acid bacteria

• Research on probiotics and fermented foods

• Laboratory and industrial microbiological studies