Modified Letheen Broth (MLEB) – Cosmetic Microbial Testing Medium with Tween 80 (250g)
Specialized Modified Letheen Broth for Cosmetic Product Microbial Analysis
This neutralization medium contains 0.7g/L lecithin and requires Tween 80 supplementation (1g per 200ml) to effectively neutralize preservatives during cosmetic product testing. The dual enzymatic digest system (5.0g/L pancreatic + 20.0g/L peptic) ensures optimal recovery of microorganisms from personal care formulations.
Key Applications:
💄 Cosmetic QC: USP/EP compliant testing for creams, lotions and makeup
🧴 Preservative Neutralization: Validated for quat, phenol and aldehyde systems
🧫 Challenge Testing: Recovery medium for preservative efficacy tests
Technical Advantages:
✅ Dual Neutralizers: 0.7g/L lecithin + Tween 80 (1g/200ml)
✅ High Nutrient: 20.0g/L meat digest supports stressed cells
✅ Antioxidant Protection: 0.1g/L sodium pyrosulfite
✅ Ready-to-Supplement: 37.8g/L base preparation
Preparation Protocol:
Suspend 37.8g in 1L purified water
Add 1g Tween 80 per 200ml medium
Autoclave at 121°C for 15 minutes
Complete Formulation (Per Liter):
Pancreatic Digest: 5.0g
Peptic Digest: 20.0g
Beef Extract: 5.0g
Yeast Extract: 2.0g
NaCl: 5.0g
Lecithin: 0.7g
Na₂S₂O₅: 0.1g
