MIO Medium for Motility-Indole-Ornithine Tests – 250g
Semi-Solid MIO Medium for Combined Biochemical Testing
Specialized low-agar formulation for simultaneous detection of motility, indole production, and ornithine decarboxylase activity in Enterobacteriaceae identification. Bromcresol purple indicator enables visual metabolic reaction monitoring.
Key Specifications:
Application: Motility-Indole-Ornithine (MIO) triple testing
Format: Semi-solid dehydrated powder (2g/L agar)
Preparation: 31.0g/L distilled water
Critical Step: Post-sterilization shaking
Solidification: Upright tubes only
pH: 6.5±0.2 (25°C)
Packaging: 250g/bottle
Shelf Life: 3 years at room temperature
Protocol Essentials:
Dissolve 31.0g in 1L water, boil to clarify
Sterilize and shake immediately
Solidify as upright tubes
Warning: Inadequate shaking causes agar sedimentation – invalidates motility reading.
