Mannitol Salt Agar (MSA) - Selective Medium for Staphylococcus aureus Isolation

Mannitol Salt Agar (MSA) – Selective Medium for Staphylococcus aureus Isolation

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Premium Mannitol Salt Agar – Gold Standard for Staphylococcal Detection

Precisely isolate and identify Staphylococcus aureus with our selective MSA medium – the definitive choice for food safety testing, clinical diagnostics, and pharmaceutical quality control. Formulated with 7.5% sodium chloride and phenol red indicator, this differential medium delivers unmistakable colony morphology for reliable pathogen identification per FDA BAM Chapter 12 and ISO 6888 standards.

Critical Applications:

  • ✔️ Food Safety Testing – Dairy/meat S. aureus enumeration

  • ✔️ Clinical Diagnostics – Wound infection identification

  • ✔️ Pharmaceutical QC – Raw material contamination screening

  • ✔️ Environmental Monitoring – Surface contact plates

  • ✔️ Cosmetic Microbiology – Preservation efficacy testing

Differential Formula Components (per liter):

| Component | Quantity |

|————————————|———-|

| Pancreatic Digest of Casein | 5.0g |

| Peptic Digest of Animal Tissue | 5.0g |

| Beef Extract | 1.0g |

| D-Mannitol | 10.0g |

| Sodium Chloride | 75.0g |

| Phenol Red | 0.025g |

| Purified Agar | 15.0g |

pH: 7.4 ± 0.2 (25°C)

Validated Preparation Protocol:

  1. Dissolve 111.0g in 1L purified water

  2. Boil with stirring until complete dissolution

  3. Autoclave: 121°C/15 min or 115°C/30 min

  4. Swirl gently post-sterilization

  5. Pour Plates at 45-50°C

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