M9 Minimal Salt (5× Concentrate) for Recombinant E. coli Culture, 250g

M9 Minimal Salt, 5× Concentrate is a dehydrated medium used for preparing M9 minimal salt medium for recombinant Escherichia coli culture. This formulation requires supplementation with carbon, magnesium, calcium, or amino acid sources to achieve complete medium preparation.

• Specification: 250g/bottle

• Storage: Room temperature

• Shelf Life: 3 years

Formula per liter (5×):

• Disodium Phosphate (anhydrous): 33.9 g

• Monopotassium Phosphate: 15.0 g

• Sodium Chloride: 2.5 g

• Ammonium Chloride: 5.0 g

• pH 6.8 ± 0.2 (25℃)

Preparation: Dissolve 56.4 g of powder in 1 liter of distilled or deionized water. Sterilize by autoclaving at 121℃ for 15 minutes and allow to cool.
To prepare M9 minimal medium, dilute appropriately, then aseptically add glucose, magnesium sulfate, calcium chloride, and amino acids (or M9 medium supplement) as required.