M8 Medium – Agar-Based Medium for Actinomycete Cultivation, 250g

M8 Medium is a specialized culture medium formulated for the cultivation of Actinomycetes. It contains a rich combination of fish peptone, yeast extract, ferric citrate, salts, and agar to support selective growth of actinomycete strains. This medium is widely used in microbiology laboratories and research for isolation and cultivation studies.

Complete Formula (per liter):

• Fish Peptone: 5.0 g

• Yeast Extract: 1.0 g

• Ferric Citrate: 0.1 g

• Sodium Chloride (NaCl): 19.45 g

• Magnesium Chloride (MgCl₂): 8.8 g

• Sodium Sulfate (Na₂SO₄): 3.24 g

• Calcium Chloride (CaCl₂): 1.8 g

• Potassium Chloride (KCl): 0.55 g

• Sodium Bicarbonate (NaHCO₃): 0.16 g

• Potassium Bromide (KBr): 0.08 g

• Agar: 12.0 g

• Final pH: 7.2 ± 0.2 at 25°C

Instructions for Use:

1. Dissolve 52.18 g of medium in 1 liter of distilled or deionized water.

2. Heat to boiling for at least 1 minute until completely dissolved.

3. Dispense into suitable containers.

4. Sterilize at 121°C for 15 minutes.

5. Cool and store until use.

Packaging & Storage:

• 250 g per bottle

• Shelf life: 3 years

• Storage: Room temperature, dry environment