M4 Medium is a microbiological culture medium designed for the cultivation of actinomycetes in laboratory environments. The formulation combines humic acid, plant peptone, buffering agents, and essential nutrients to support microbial growth and colony development.
This powder microbiological culture medium is suitable for preparing agar plates used in actinomycete cultivation and microbial research. The medium provides a stable nutrient environment for laboratory microbiology studies and environmental microorganism investigations.
The product is supplied as a dehydrated powder packaged in a 250 g bottle, allowing convenient preparation of agar medium in microbiology laboratories.
Antibiotics may be added as required to increase microbial selectivity when necessary.
Key Features
- Designed for actinomycetes cultivation
- Microbiological agar culture medium powder
- Suitable for Streptomyces and actinomycete research
- Stable formulation for laboratory use
- Easy preparation and sterilization
- 250 g laboratory packaging
- Shelf life up to 3 years
Applications
- Cultivation of actinomycetes
- Streptomyces culture
- Environmental microbiology research
- Microbial laboratory studies
- Academic microbiology research
Medium Composition
Composition (per liter)
Humic Acid — 1.0 g
Plant Peptone — 0.5 g
MOPS — 10 mmol
Betaine — 1.25 g
Sodium Pyruvate — 1.25 g
Sodium Chloride — 10.0 g
Agar — 12.0 g
Final pH: 7.2 ± 0.2 (25°C)
Directions for Use
- Suspend 26.2 g of the medium in 1 liter of distilled or deionized water.
- Heat and boil for at least 1 minute until completely dissolved.
- Dispense into appropriate containers.
- Sterilize by autoclaving at 121°C for 15 minutes.
- Allow the medium to cool before pouring agar plates if required.
Antibiotics may be added as needed to enhance selectivity.
Product Specifications
| Item | Description |
|---|---|
| Product Name | M4 Medium |
| Format | Powder |
| Package | 250 g / bottle |
| Storage | Room temperature |
| Shelf Life | 3 years |
| Application | Actinomycetes cultivation |