M3 Medium for Actinomycetes Culture | Microbiological Agar Culture Medium Powder 250g

M3 Medium is a microbiological culture medium developed for the cultivation of actinomycetes and related microorganisms in laboratory environments. The formulation contains balanced nutrients that support microbial growth and colony development, making it suitable for routine microbiology research and actinomycete culture studies.

This microbiological agar medium is supplied as a dehydrated powder, allowing convenient preparation in laboratories. After dissolution and sterilization, the medium can be used to prepare agar plates suitable for microbial culture procedures.

M3 Medium is widely used in microbiology laboratories, environmental microbial research, and academic studies involving actinomycetes and related microorganisms.

Antibiotics may be added when increased microbial selectivity is required.

Key Features

• Designed for actinomycetes cultivation
• High-quality microbiological agar culture medium
• Supplied as dehydrated powder
• Suitable for laboratory microbial culture
• Easy preparation and sterilization
• 250 g laboratory packaging
• 3-year shelf life

Applications

• Cultivation of actinomycetes
• Streptomyces research and microbial studies
• Environmental microbiology research
• Academic microbiology laboratories
• Microbial culture experiments

Medium Composition

Composition (per liter)

Peptone — 1.0 g
Beef Extract Powder — 0.5 g
Betaine — 1.25 g
Sodium Pyruvate — 1.25 g
Sodium Chloride — 10.0 g
Agar — 12.0 g

Final pH: 7.2 ± 0.2 (25°C)

Directions for Use

1. Suspend 26.0 g of the medium in 1 liter of distilled or deionized water.
2. Heat and maintain boiling for at least 1 minute until completely dissolved.
3. Dispense into suitable containers.
4. Sterilize by autoclaving at 121°C for 15 minutes.
5. Allow the medium to cool before pouring plates if agar plates are required.

Antibiotics may be added if increased selectivity is required.

Product Specifications