M2 Medium is a microbiological culture medium formulated for the isolation and cultivation of actinomycetes in laboratory environments. The composition provides a balanced combination of nutrients and mineral salts suitable for microbial growth and colony development.
This microbiological agar medium contains chitin and essential components that help create a suitable environment for actinomycete growth during laboratory isolation procedures.
Supplied as a dehydrated powder, the medium can be easily prepared by dissolving in distilled or deionized water followed by sterilization. It is widely used in microbiology laboratories, environmental microbial studies, and academic research involving actinomycetes and related microorganisms.
Key Features
- Designed for actinomycetes isolation and cultivation
- High-quality microbiological agar culture medium
- Supplied as dehydrated powder medium
- Suitable for microbial culture in laboratory research
- Easy preparation and sterilization
- 250 g laboratory bottle packaging
- Stable storage with 3-year shelf life
Applications
- Isolation of actinomycetes
- Cultivation of Streptomyces and related microorganisms
- Environmental microbiology studies
- Soil microorganism research
- Academic microbiology laboratories
Medium Composition
Composition (per liter)
Chitin — 1.0 g
Sodium Pyruvate — 1.25 g
Magnesium Sulfate Heptahydrate (MgSO4·7H2O) — 0.5 g
Dipotassium Phosphate (K2HPO4) — 0.7 g
Ferrous Sulfate Heptahydrate (FeSO4·7H2O) — 0.01 g
Sodium Chloride (NaCl) — 10.0 g
Agar — 12.0 g
Final pH: 7.2 ± 0.2 (25°C)
Directions for Use
- Suspend 25.5 g of the medium in 1 liter of distilled or deionized water.
- Heat and boil for at least 1 minute until the medium is completely dissolved.
- Dispense into suitable containers.
- Sterilize by autoclaving at 121°C for 15 minutes.
- Allow the medium to cool before use or before pouring agar plates if required.
Product Specifications
| Item | Description |
|---|---|
| Product Name | M2 Medium |
| Format | Powder |
| Package | 250 g / bottle |
| Storage | Room temperature |
| Shelf Life | 3 years |
| Application | Actinomycetes isolation and cultivation |