M17 Broth – Medium for Detection of Lactic Acid Bacteria in Milk & Dairy Products

M17 Broth is a high-quality liquid medium designed for the detection and enumeration of lactic acid bacteria in milk and dairy products. This medium requires supplementation with a 10% lactose solution before use and is widely used in dairy microbiology laboratories.

Product Information:

• Name: M17 Broth

• Form: Dehydrated powder

• Pack Size: 250g per bottle

• Storage: Room temperature

• Shelf Life: 3 years

Composition (per liter):

• Tryptic Digest of Casein – 2.5 g

• Peptic Digest of Meat – 2.5 g

• Papain Digest of Soya – 5.0 g

• Yeast Extract – 2.5 g

• Meat Extract – 5.0 g

• β-Glycerophosphate (disodium salt) – 19.0 g

• Ascorbic Acid – 0.5 g

• Magnesium Sulfate Heptahydrate – 0.25 g

• pH: 6.9 ± 0.2 (25℃)

Preparation Instructions:

1. Dissolve 3.73 g of the powder in 95 mL distilled or deionized water, heat and boil until fully dissolved.

2. Dispense and sterilize by autoclaving at 121℃ for 15 minutes.

3. Cool to 50℃, then aseptically add 5 mL sterile 10% lactose solution per 100 mL medium and mix gently.

4. Ready for detection of lactic acid bacteria in milk and dairy products.