M17 Agar – Medium for Detection of Lactic Acid Bacteria in Milk & Dairy Products

M17 Agar is a high-quality medium designed for the detection and enumeration of lactic acid bacteria in milk and dairy products. This medium requires supplementation with 10% lactose solution before use and is widely used in dairy microbiology laboratories.

Product Information:

• Name: M17 Agar

• Form: Dehydrated powder

• Pack Size: 250g per bottle

• Storage: Room temperature

• Shelf Life: 3 years

Composition (per liter):

• Tryptic Digest of Casein – 2.5 g

• Peptic Digest of Meat – 2.5 g

• Papain Digest of Soya – 5.0 g

• Yeast Extract – 2.5 g

• Meat Extract – 5.0 g

• β-Glycerophosphate (disodium salt) – 19.0 g

• Ascorbic Acid – 0.5 g

• Magnesium Sulfate Heptahydrate – 0.25 g

• Agar – 11.0 g

• pH: 6.9 ± 0.2 (25℃)

Preparation Instructions:

1. Dissolve 4.83 g of the powder in 95 mL distilled or deionized water, heat and boil until fully dissolved.

2. Dispense and sterilize by autoclaving at 121℃ for 15 minutes.

3. Cool to 55℃, then aseptically add 5 mL sterile 10% lactose solution per 100 mL medium and mix gently.

4. Pour plates and allow to solidify; ready for detection of lactic acid bacteria in milk and dairy products.