Product Specifications
- Product Name: m-Pseudomonas aeruginosa Agar
- Alternative Names: PA Agar / m-PA Agar / m-Pseudomonas aeruginosa Agar
- Format: Powder Medium
- Net Weight: 250 g / bottle
- Storage: Store in a cool and dry place at room temperature
- Shelf Life: 3 years
- Application: Detection and enumeration of Pseudomonas aeruginosa in water samples
Composition (g/L)
Agar ………………………………………….. 15.0
Sodium Thiosulfate (Na₂S₂O₃) ………….. 6.8
L-Lysine Hydrochloride ……………………. 5.0
Sodium Chloride …………………………….. 5.0
Xylose ………………………………………….. 2.5
Yeast Extract ………………………………… 2.0
Lactose ………………………………………… 1.25
Sucrose ………………………………………… 1.25
Ferric Ammonium Citrate ………………… 0.8
Phenol Red ……………………………………. 0.08
pH 7.1 ± 0.2 (25°C)
Preparation Instructions
- Weigh 39.7 g of the medium powder.
- Dissolve in 1000 mL distilled or deionized water.
- Heat and boil for at least 1 minute to completely dissolve the medium.
- Dispense into suitable containers.
- Sterilize at 121°C for 15 minutes.
- After sterilization, mix well to prevent agar sedimentation and cool to 50–55°C.
- Under aseptic conditions, add the following supplements to each 100 mL of medium:
- Sodium sulfapyridine solution
- m-PA agar supplement
- Nalidixic acid solution
- Kanamycin solution
- Mix gently and pour into sterile Petri dishes.
Applications
- Detection of Pseudomonas aeruginosa
- Water microbiology testing
- Membrane filtration bacterial enumeration
- Drinking water quality testing
- Environmental water monitoring laboratories
- Public health laboratories