Product Specifications
- Product Name: M-PA-C Agar
- Alternative Names: PA-C Agar / mPA-C Agar
- Format: Powder Medium
- Net Weight: 250 g / bottle
- Storage: Store in a cool and dry place at room temperature
- Shelf Life: 3 years
- Application: Detection and enumeration of Pseudomonas aeruginosa in water
Composition (g/L)
Yeast Extract …………………………………. 2.0
L-Lysine HCl ……………………………………. 5.0
Sodium Chloride ……………………………….. 5.0
Xylose ……………………………………………. 1.25
Sucrose ………………………………………….. 1.25
Lactose ………………………………………….. 1.25
Phenol Red ……………………………………… 0.08
Ferric Ammonium Citrate …………………… 0.8
Sodium Thiosulfate ………………………….. 5.0
Magnesium Sulfate ……………………………. 1.5
Kanamycin ………………………………………. 8.0 mg
Nalidixic Acid ………………………………….. 37.0 mg
Agar ……………………………………………… 12.0
pH 7.2 ± 0.1 (25°C)
Preparation Instructions
- Weigh 35.0 g of the medium powder.
- Dissolve in 1000 mL distilled or deionized water.
- Heat and boil for 1 minute until completely dissolved.
- Pour into sterile Petri dishes and allow to solidify before use.
Important Notes
- This medium must not be autoclaved.
- Due to the relatively high magnesium sulfate content, granular insoluble particles may appear after boiling. This is normal; mix well before pouring plates.
Applications
- Detection of Pseudomonas aeruginosa
- Enumeration of bacteria in water samples
- Environmental microbiology testing
- Drinking water quality monitoring
- Public health laboratories
- Research laboratories