m-HPC Agar Base Medium for Heterotrophic Plate Count

m-HPC Agar Base Medium is a dehydrated culture medium designed for the enumeration of heterotrophic bacteria in water and environmental samples. It is widely used in drinking water analysis, bottled water testing, environmental monitoring, and microbiological research.

⚠ For laboratory research and industrial testing use only. Not for clinical or therapeutic use.

🔬 Intended Use

• Heterotrophic plate count (HPC) testing

• Drinking water and bottled water analysis

• Environmental and industrial water monitoring

• Microbiological research and quality control

📦 Product Specifications

• Product Name: m-HPC Agar Base Medium

• English Name: Heterotrophic Plate Count Agar

• Physical Form: Dehydrated powder

• Package Size: 250 g / bottle

• Storage Condition: Room temperature (RT)

• Shelf Life: 3 years

⚗️ Medium Preparation (General Guidelines)

1️⃣ Dissolution

• Add the required amount of dehydrated medium to distilled or deionized water

• Allow to stand for 10–15 minutes to facilitate hydration

• Stir, shake, or swirl gently to promote dissolution

• If necessary, heat gently with constant stirring until fully dissolved

• Avoid excessive heating or prolonged high temperatures to prevent nutrient degradation

2️⃣ pH Adjustment

• The pH of the medium should be measured at 25 ± 2 °C

• Adjust pH using sterile solutions if required:

  • 1 mol/L sodium hydroxide

  • 10% sodium carbonate

  • 1 mol/L hydrochloric acid

• Note: The pH after sterilization may differ slightly from pre-sterilization values.

  • It is common practice to adjust the pH approximately 0.2 units higher before autoclaving

• Although dehydrated media are pre-adjusted, pH verification before use is recommended

3️⃣ Clarification

• After pH adjustment, heat and filter if necessary to obtain a clear medium