Lowenstein-Jensen Medium Base – 250g for Mycobacteria Isolation

Lowenstein-Jensen Medium Base is specifically formulated for the selective isolation and identification of Mycobacterium species, including Mycobacterium tuberculosis. The medium contains asparagine, potato flour, and malachite green to support optimal growth while suppressing contaminants.

Product Information

• Product Name: Lowenstein-Jensen Medium Base

• Pack Size: 250g per bottle

• Form: Dehydrated powder

• Storage: Room temperature

• Shelf Life: 3 years

Applications

• Selective isolation of Mycobacterium species

• Cultivation of Mycobacterium tuberculosis

• Laboratory and clinical microbiology research

Composition (per liter)

• Asparagine – 3.6g

• Monopotassium Phosphate – 2.4g

• Magnesium Sulfate – 0.24g

• Magnesium Citrate – 0.6g

• Potato Flour – 30.0g

• Malachite Green – 0.4g

Preparation Instructions

1. Dissolve 37.4g of the medium in 600ml distilled or deionized water containing 12ml glycerol.

2. Sterilize at 121℃ for 15 minutes.

3. Cool to approximately 50℃.

4. Aseptically add 1000ml whole egg and mix gently, avoiding foam formation.

5. Let stand for 1 hour, then dispense into tubes.

6. Place tubes at an angle in a serum coagulator and gently heat at 80–85℃ for 1–1.5 hours to solidify the slant.

7. Medium is ready for Mycobacterium isolation and cultivation.