Levine’s Eosin-Methylene Blue Lactose Agar (250g) – EMB Agar
Levine’s Eosin-Methylene Blue Lactose Agar (EMB Agar) is a specialized medium designed for the selective isolation of intestinal pathogens and Escherichia coli. This 250g bottle provides a high-quality formulation that supports the growth of target bacteria while inhibiting non-target microorganisms, making it ideal for microbiological testing applications.
Key Features
Primary Use: Specifically formulated for the selective isolation of intestinal pathogens and E. coli.
Formula (per liter): Contains Pancreatic Digest of Gelatin (10.0g), Dibasic Potassium Phosphate (2.0g), Lactose (10.0g), Eosin Y (0.4g), Methylene Blue (0.065g), and Agar (15.0g), with a pH of 7.1±0.2 at 25°C.
Storage: Stable at room temperature with a 3-year shelf life, ensuring consistent performance and long-term usability.
Preparation Instructions
Measure 37.5g of the medium and dissolve in 1L of distilled or deionized water (adjust quantities proportionally for larger or smaller volumes).
Heat to a boil for 1 minute to ensure complete dissolution.
Dispense and sterilize at 121°C for 15 minutes.
Cool to approximately 55°C, pour into petri dishes, and allow to cool and solidify before use.
This EMB Agar is a reliable tool for laboratories requiring accurate isolation of intestinal pathogens and E. coli.
