Lees Agar – 500g Bottle for Lactobacillus and Streptococcus Thermophilus Enumeration

Lees Agar is a specialized culture medium designed for the differentiation and enumeration of Lactobacillus and Streptococcus thermophilus in yogurt fermentation. It ensures accurate bacterial counting and supports reliable laboratory research.

Product Information

• Product Name: Lees Agar

• Pack Size: 500g per bottle

• Form: Dehydrated powder

• Storage: Room temperature

• Shelf Life: 3 years

Applications

• Differentiation of Lactobacillus and Streptococcus thermophilus

• Enumeration of lactic acid bacteria in fermented dairy products

• Yogurt fermentation quality control

• Laboratory microbiology research

Composition (per liter)

• Casein enzymic hydrolysate – 10.0g

• Yeast extract – 10.0g

• Lactose – 5.0g

• Sucrose – 5.0g

• Calcium carbonate – 3.0g

• Dipotassium phosphate – 0.5g

• Bromocresol purple – 0.02g

• Agar – 18.0g

• pH: 7.0 ± 0.2 (25℃)

Preparation Instructions

1. Dissolve 51.52g of the medium in 1L of distilled or deionized water.

2. Heat and boil for at least 1 minute to fully dissolve.

3. Dispense into containers and sterilize at 121℃ for 15 minutes.

4. Cool the medium to approximately 50℃ before preparing plates.

5. Gently shake to evenly distribute calcium carbonate insoluble particles throughout the plates.

6. Plates are ready for use in bacterial enumeration.