
Lauryl Sulfate Tryptose Broth (LST) – Medium for E. coli and Coliform Testing, 250g
Lauryl Sulfate Tryptose Broth (LST) is a selective medium used for detection and enumeration of Escherichia coli and coliform bacteria. Supplied in 250g bottles, it is stable at room temperature with a shelf life of 3 years, suitable for microbiology laboratories performing water, food, and environmental testing.
Composition (per liter):
Pancreatic Digest of Casein: 20.0g
Sodium Chloride: 5.0g
Lactose: 5.0g
Dipotassium Phosphate: 2.75g
Monopotassium Phosphate: 2.75g
SDS (Sodium Lauryl Sulfate): 0.1g
pH: 6.8 ± 0.2 (25°C)
Usage Instructions:
Dissolve 35.6g of medium in 1L of distilled or deionized water. Heat to boiling until completely dissolved. Dispense and sterilize at 121°C for 15 minutes. Cool and use for testing E. coli and coliform bacteria.