Lactophenol Cotton Blue Stain for Fungal Morphology
Lactophenol Cotton Blue Stain is a specialized laboratory reagent designed for clear visualization of fungal structures, including spores and hyphae, under microscopic examination. It provides distinct blue staining of fungal components, making it an essential tool for microbial laboratory analysis of fungi.
Key Specifications:
Packaging: 5ml/vial × 4 vials/box
Storage Condition: Room temperature, protected from light
Shelf Life: 1 year
Intended Use:
Specifically formulated for staining and observing fungal morphology, enabling clear identification of spores, hyphae, and asci in laboratory settings.
How to Use:
Prepare a smear: Place 1-3 drops of lactophenol cotton blue stain in the center of a slide.
Using a sterile inoculating loop or dissecting needle, take a small amount of mycelium with spores from the edge of a mold colony, place it in the stain, and disperse the mycelium for even distribution. Note: Mold culture time should not be excessively long, as excessive spores may interfere with staining and observation of hyphae and asci.
Cover with a clean coverslip, press gently to make a flattened preparation, taking care to avoid air bubbles.
Observe fungal morphology using a light microscope under low, high, or oil immersion magnification.
Result interpretation: Fungal components (especially Aspergillus fumigatus) such as spores and hyphae appear blue, bright blue, dark blue, or navy blue.