Lactobacillus Broth Medium – Nutrient Broth for Lactobacillus, 250g

Lactobacillus Broth Medium is a nutritive liquid culture medium specifically designed for the growth and propagation of Lactobacillus species. This medium is suitable for microbiological research, quality control in dairy and fermented foods, and probiotic cultivation.

Product Information

• Product Name: Lactobacillus Broth Medium

• Form: Dehydrated powder for broth

• Pack Size: 250g per bottle

• Storage: Room temperature

• Shelf Life: 2 years

Medium Composition (per liter)

• Peptone Milk – 15.0 g

• Yeast Extract – 5.0 g

• Glucose – 10.0 g

• Tomato Juice Powder (equivalent to 100 ml tomato juice) – 2.5 g

• Monopotassium Phosphate – 2.0 g

• Polysorbate 80 – 1.0 g

• pH: 6.8 ± 0.2 (25℃)

Preparation Instructions

1. Dissolve 35.5 g of the medium in 1 liter of distilled or deionized water.

2. Heat gently until fully dissolved.

3. Dispense into test tubes or other vessels.

4. Sterilize at 121℃ for 15 minutes.

5. Cool and store appropriately.

Notes

• The medium is opaque due to insoluble components from tomato juice powder.

• Polysorbate 80 may cause the powder to be slightly moist and prone to clumping, but this does not affect usability.

Applications

• Cultivation of Lactobacillus species

• Microbiological testing in dairy, fermented foods, and probiotics

• Probiotic strain propagation and research