Product Overview
King’s B Medium, also known as King B Agar, is a microbiological culture medium designed for the fluorescence identification of Pseudomonas aeruginosa.
This dehydrated medium supports bacterial growth and allows observation of fluorescence characteristics that assist in microbial identification procedures. The medium is widely used in microbiology laboratories for bacterial identification, environmental microbiology studies, and research involving Pseudomonas species.
The powder medium requires dissolution in purified water and the addition of glycerol prior to sterilization and culture preparation.
Product Specifications
| Parameter | Description |
|---|---|
| Product Name | King’s B Medium |
| Alternative Name | King B Agar |
| Application | Fluorescence identification of Pseudomonas aeruginosa |
| Packaging | 250 g / bottle |
| Storage | Room temperature |
| Shelf Life | 3 years |
| Form | Dehydrated powder |
Applications
King’s B Medium is commonly used for:
- Fluorescence identification of Pseudomonas aeruginosa
- Microbiology laboratory testing
- Environmental microbiology studies
- Research involving Pseudomonas species
- Bacterial identification in microbiological analysis
Medium Composition (per liter)
Peptone — 20.0 g
Dipotassium Phosphate — 1.5 g
Magnesium Sulfate (7H₂O) — 1.5 g
Agar — 15.0 g
Final pH: 7.2 ± 0.2 (25°C)
Preparation Instructions
- Suspend 38.0 g of the medium in 1 liter of distilled or deionized water.
- Add 1 ml glycerol per 100 ml medium.
- Heat with mixing and boil for at least 1 minute until the agar is completely dissolved.
- Dispense into test tubes or suitable containers.
- Sterilize by autoclaving at 121°C for 15 minutes.
- Mix thoroughly and allow the medium to solidify as slants if required.
Note:
Small amounts of inorganic salt precipitate may occasionally appear and do not affect the performance of the medium.