KCN Test Base Medium (Cyanide-Free) for Bacterial Identification
Specialized Base for Potassium Cyanide Tolerance Testing
This cyanide-free base medium requires addition of sterile KCN solution to create selective media for differentiating cyanide-resistant bacteria like Salmonella and Proteus.
Critical Safety Note:
△ Must add 0.5% sterile KCN solution post-sterilization
△ For trained laboratory personnel only
Key Features:
Precisely buffered phosphate system (pH 7.2±0.2)
Room temperature stable for 3 years
Optimized peptone-to-electrolyte ratio
Complies with standard KCN test protocols
Technical Parameters:
▶ Formula (per liter):
Peptone 10.0g | NaCl 5.0g
KH₂PO₄ 0.225g | Na₂HPO₄ 5.64g
▶ pH: 7.2±0.2 (25°C)
▶ Preparation:
20.9g/L → 121°C/15min sterilization
Cool <50°C → add 20ml 0.5% sterile KCN
Applications:
Enterobacteriaceae identification
Clinical isolate characterization
Microbial tolerance studies
