ISP Medium 5 (Glycerol Asparagine Agar Base) – Streptomyces Cultivation & Identification Agar

ISP Medium 5 (Glycerol Asparagine Agar Base) is designed for the cultivation and identification of Streptomyces species as part of the International Streptomyces Project (ISP).
This agar base requires the addition of Glycerol (10 g per liter) for complete preparation. It is widely used in microbiological laboratories for taxonomic studies and characterization of actinomycetes.

• Form: Dehydrated culture medium

• Packaging: 250 g/bottle

• Storage: Room temperature

• Shelf Life: 3 years

✅ Product Formula (per liter)

• L-asparagine …………………………………. 1.0 g

• Di-potassium hydrogen phosphate (K₂HPO₄) ………… 1.0 g

• Ferrous sulphate (FeSO₄) ………………………. 1.0 mg

• Manganese chloride (MnCl₂) ……………………. 1.0 mg

• Zinc sulphate (ZnSO₄) ………………………… 1.0 mg

• Agar ………………………………………… 15.0 g

• Final pH: 7.2 ± 0.2 (at 25℃)

✅ Instructions for Use

1. Suspend 17.0 g of the medium base and 10 g of glycerol in 1 liter of deionized water or 30% natural seawater.

2. Heat to boiling until completely dissolved.

3. Dispense into suitable containers.

4. Sterilize by autoclaving at 121℃ for 15 minutes.

5. After sterilization, shake well to prevent agar from settling at the bottom before solidification.