Inositol Assay Medium – Culture Medium for Myo-Inositol Determination, 250g

Inositol Assay Medium is a specialized microbiological medium formulated for the determination of inositol (myo-inositol, Vitamin B8) in food, nutritional, and research applications. Its balanced nutrient composition supports accurate and reproducible assay results.

Complete Formula (per liter):

• Glucose: 100.0 g

• Potassium Citrate: 10.0 g

• Citric Acid: 2.0 g

• KH2PO4: 1.1 g

• KCl: 0.85 g

• MgSO4: 0.25 g

• CaCl2: 0.25 g

• MnSO4: 0.05 g

• FeCl2: 0.05 g

• DL-Tryptophan: 0.08 g

• L-Cystine: 0.1 g

• L-Isoleucine: 0.5 g

• L-Leucine: 0.5 g

• L-Lysine: 0.5 g

• L-Methionine: 0.2 g

• DL-Phenylalanine: 0.2 g

• L-Tyrosine: 0.2 g

• L-Aspartic Acid: 0.8 g

• DL-Aspartic Acid: 0.2 g

• DL-Serine: 0.1 g

• Glycine: 0.2 g

• DL-Threonine: 0.4 g

• L-Valine: 0.5 g

• L-Histidine: 0.124 g

• L-Proline: 0.2 g

• DL-Alanine: 0.4 g

• L-Glutamic Acid: 0.6 g

• L-Arginine: 0.48 g

• Thiamine Hydrochloride: 500 µg

• Biotin: 16 µg

• Calcium Pantothenate: 0.005 g

• Pyridoxine Hydrochloride: 0.001 g

• Final pH: 5.2 ± 0.2 (25°C)

Instructions for Use:

1. Weigh 12.1 g of the medium.

2. Dissolve in 100 ml distilled or deionized water.

3. Heat to boiling until completely dissolved.

4. Cool and dispense into suitable containers.

5. Store the prepared medium for immediate assay use.