Humic Acid HV Medium – Agar-Based Medium for Actinomycete Cultivation, 250g

Humic Acid HV Medium (HV Agar) is a selective culture medium formulated for the cultivation of Actinomycetes. Enriched with humic acid, essential minerals, vitamins, and agar, this medium provides a stable environment for the growth and differentiation of actinomycete strains in microbiological and research applications.

Complete Formula (per liter):

• Humic Acid: 1.0 g

• Calcium Carbonate (CaCO₃): 0.02 g

• Disodium Phosphate (Na₂HPO₄): 0.5 g

• Magnesium Sulfate Heptahydrate (MgSO₄·7H₂O): 0.05 g

• Potassium Chloride (KCl): 1.7 g

• Ferrous Sulfate Heptahydrate (FeSO₄·7H₂O): 0.01 g

• Vitamin B1 (Thiamine): 0.5 mg

• Vitamin B2 (Riboflavin): 0.5 mg

• Vitamin B6 (Pyridoxine): 0.5 mg

• Nicotinic Acid: 0.5 mg

• Inositol: 0.5 mg

• Pantothenic Acid (Vitamin B5): 0.5 mg

• Biotin: 0.25 mg

• p-Aminobenzoic Acid (PABA): 0.5 mg

• Naphthionic Acid: 20.0 mg

• Agar: 20.0 g

• Final pH: 7.2 ± 0.2 at 25°C

Instructions for Use:

1. Dissolve 23.3 g of the medium in 1 liter of distilled or deionized water.

2. Heat and stir, boiling for at least 1 minute until agar is completely dissolved.

3. Dispense into containers.

4. Sterilize at 121°C for 15 minutes.

5. Cool and store until use.

Packaging & Storage:

• 250 g per bottle

• Shelf life: 3 years

• Storage: Room temperature, dry environment