Hektoen Enteric Agar Medium (250g) - HEA Medium for selective isolation of intestinal bacteria

Hektoen Enteric Agar Medium (250g) – HEA Medium

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Hektoen Enteric Agar Medium (HEA Medium, also known as HEK Agar Medium) is a specialized formulation designed for the selective isolation of intestinal bacteria. This 250g bottle provides a high-quality medium optimized to support the growth of target intestinal bacteria while inhibiting non-target microorganisms, making it a reliable tool for microbiological testing.

Key Features

  • Primary Use: Specifically formulated for the selective isolation of intestinal bacteria.

  • Formula (per liter): Contains Protease Peptone (12.0g), Yeast Extract (3.0g), Sucrose (12.0g), Lactose (12.0g), Salicin (2.0g), Bile Salts No.3 (9.0g), Sodium Chloride (5.0g), Sodium Thiosulfate (5.0g), Ferric Ammonium Citrate (1.5g), Acid Fuchsin (0.1g), Bromthymol Blue (0.065g), and Agar (14.0g), with a pH of 7.5±0.1 at 25°C.

  • Storage: Stable at room temperature with a 3-year shelf life, ensuring consistent performance and long-term usability.

Preparation Instructions

  1. Measure 75.6g of the medium and dissolve in 1L of distilled or deionized water (adjust quantities proportionally for larger or smaller volumes).

  2. Heat to a boil for 1 minute to ensure complete dissolution.

  3. Pour directly into petri dishes and allow to solidify before use.

This HEA Medium is ideal for laboratories requiring accurate selective isolation of intestinal bacteria.

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